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. 2013 Sep 30;42(1):526–533. doi: 10.1093/nar/gkt867

Figure 2.

Figure 2.

Binding of Rad51-ssDNA non-homologous filaments to dsDNA. (A) Extension versus time binding profiles for dsDNA pulled 3′5′ (red) in the presence of Rad51-ssDNA filaments 700 bases in length and negative controls with 10 nM Rad51 (black) and ssDNA and no protein (gray); force range 54–58 pN. (B) Frequency of extensions after 120 s of constant force for forces between 54 and 58 pN in the presence of Rad51-ssDNA filaments. (C) Frequency of extensions or the controls after 120 s of constant force for forces between 54 and 58 pN in the presence of 10 nM Rad51 (black) and ssDNA (gray). Filaments are 700-nt long obtained from pcDNA3 non-homolog to lambda phage dsDNA.