Figure 5.

In vitro cotranscriptional and in vivo structure probing. (a) RNA was transcribed in vitro by E. coli RNAP for 5 min before the addition of DMS. (b) Cells containing plasmids carrying the locus P_alx-PRE-alx' were grown in LB and treated with DMS for 5 min before RNA extraction. Reverse transcription of DMS modified (+) and unmodified (−) RNA. Modifications indicative of the active structure are marked as ‘active’, whereas modifications indicative of the inactive structure are marked as ‘inactive’. A summary of cotranscriptional structure probing data is displayed in Figure 2.