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. 2013 Sep 27;42(1):349–358. doi: 10.1093/nar/gkt879

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — SWI5-SFR1 but not RAD51AP1 mediates ADP–ATP exchange of RAD51 filament. (A) Schematic of the filter-binding assay to monitor ADP–ATP exchange within the RAD51-ssDNA presynaptic filament. (B) SWI5-SFR1 facilitates ADP–ATP exchange of RAD51 filament in a dosage-dependent manner. (C) There is no enhancement of nucleotide exchange by SWI5-SFR1 on the omission of ssDNA. (D) RAD51AP1 lacks the ability to facilitate ADP–ATP exchange within the RAD51 presynaptic filament. (B–D) Error bars represent the standard deviation (±SD) calculated based on at least three independent experiments. Symbol: S5S1, SWI5-SFR1.