Table 1. Detailed Statistics of Nine Constructs from Assembling One dsDNA Insert and De Novo Synthesis of a Sequence Between 30–255 bpa.
| construct | hit rate colony PCR (%) | sequencing result mutated/failed/correct | correct clones (%) | DNA synthesis length | errors total | error per bp | insertions/deletions/mutations |
|---|---|---|---|---|---|---|---|
| 13 | 58.33 | 3/0/1 | 25.00 | 87 | 5 | 0.057 | 4/1/0 |
| 15 | 41.67 | 2/0/2 | 50.00 | 114 | 2 | 0.017 | 2/0/0 |
| 22 | 83.33 | 1/0/3 | 75.00 | 96 | 2 | 0.020 | 2/0/0 |
| 19 | 66.67 | 1/1/2 | 50.00 | 69 | 1 | 0.014 | 1/0/0 |
| 30 | 66.67 | 2/0/2 | 50.00 | 96 | 3 | 0.031 | 1/0/2 |
| 31 | 83.33 | 3/0/1 | 25.00 | 96 | 5 | 0.052 | 3/0/2 |
| 36 | 41.67 | 0/0/4 | 100.00 | 46 | 0 | 0 | 0/0/0 |
| 46 | 75.00 | 1/0/3 | 75.00 | 73 | 1 | 0.013 | 0/0/1 |
| 41 | 58.33 | 2/1/1 | 25.00 | 52 | 9 | 0.173 | 1/8/0 |
| total | 63 | 15/2/19 | 52 | 729 | 21 | 0.03 | 14/2/5 |
All nine constructs were obtained in the last round of transformation. The identifiers of the constructs are given in the first column (refer to Supporting Information Table 1). The percentage of positive clones from 12 colonies when screened by PCR is given in column two. The result from four positive clones sent to sequencing is given in the third column. Where indicated, failed corresponds to no sequencing result. The DNA synthesis length column specifies the length of sequence synthesized by oligonucleotides in the corresponding construct. The errors total column indicates the number of all errors found per construct, including multiple errors in one construct. The next column reports the error rate per synthesized base pair: the dsDNA parts were not counted towards this error rate. The last column reports the type of errors that were found. The bottom row reports the average for columns 2, 4, and 7 as well as the sum for all other columns.