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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Cancer Lett. 2013 Sep 15;343(1):10.1016/j.canlet.2013.09.014. doi: 10.1016/j.canlet.2013.09.014

Figure 4. TGLI1-carrying xenografts of GBM express high levels of VEGF-A.

Figure 4

A,B, TGLI1-carrying GBM xenografts contained a higher level of VEGF-A than those with GLI1. Panel A, RT-PCR. Panel B, WB. Results of six representative tumors are shown. CD24 is highly expressed in TGLI1-carrying tumors but not in those with GLI1, which is in agreement with our previous report [17].

C,D, TGLI1-carrying xenografts expressed more VEGF-A than those with GLI1, as shown by IHC. Six tumors per group were analyzed. Student t-test was conducted to determine the p-value. Panel D shows two representative tumors. TGLI1- and GLI1-positive nuclei are marked by brown signals.

E,F, TGLI1-expressing GBM xenografts contained a higher propensity to promote in vitro angiogenesis of HBMEC cells as shown by the tubule formation assay. Total proteins extracted from the xenografts were added to endothelial cells. Three independent experiments were conducted and the student t-test was conducted to determine p-values.