Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Head Neck. 2013 Apr 30;36(1):10.1002/hed.23267. doi: 10.1002/hed.23267

Figure 3.

Figure 3

The Sequenom™ assay interrogates predefined gene single nucleotide polymorphisms (A.). The assays begins with and initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer (ddATP*, ddGTP*, ddCTP*, ddTTP*) which anneals immediately upstream of the polymorphic site of interest (B.). Allele-specific products with distinct masses (C.). Using MALDI-TOF mass spectrometry (Matrix-assisted laser desorption/ionization – time of flight) the distinct mass of the extended primer identifies the SNP allele (D.).