Figure 6.

Antcin C upregulates antioxidant genes in AAPH-challenged liver tissues. Mice were pretreated with antcin C (25–200 mg/kg) or silymarin (100 mg/kg) for 5 days, and then challenged with a single dose of AAPH (80 mg/kg). Mice were sacrificed, and the liver sections were subjected to immunohistochemistry to analyse the protein expression levels of HO-1, NQO-1, and Nrf2 using specific antibodies. The relative intensity of antibody positive staining cells was quantified by Image-Pro Plus software. The results are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 were considered significant for sample versus control.