Table 1.
Bacterial strains used as positive and negative controls in a multiplex polymerase chain reaction designed for detection of Clostridium perfringens genes netB and tpeL.
| Strain ID number | Species | netB | tpeL | Relevance | Source |
|---|---|---|---|---|---|
| C103-99 | Clostridium perfringens | + | + | Positive control | Necrotic enteritis |
| JGS-1870 | Clostridium perfringens | + | + | Positive control | Necrotic enteritis |
| JGS-4140 | Clostridium perfringens | + | + | Positive control | Necrotic enteritis |
| 232-2 | Clostridium bifermentans | − | − | Negative control | Poultry litter |
| 259-14 | Bacteroides caccae | − | − | Negative control | Poultry litter |
| 250-4 | Wolinella sp. | − | − | Negative control | Poultry litter |