Table 2.
Specifications of primers used in a multiplex PCR for the detection of netB and tpeL.
| Primer ID | Sequence | Target gene | Product length |
|---|---|---|---|
| netB5F | CGCTTCACATAAAGGTTGGAAGGC | netB | 316 |
| netB5R | TCCAGCACCAGCAGTTTTTCCT |
||
| AKP80* | ATATAGAGTCAAGCAGTGGAG | tpeL | 466 |
| AKP81* | GGAATACCACTTGATATACCTG |
*Primers previously published by Keyburn et al., 2010 [7].