Figure 5.

Immunofluorescence analysis of dystrophin protein in the diaphragm of wild type (WT), untreated (mdx), and alginate-ZM2-M23D orally treated (treated) mdx mice. The sections were labeled with antidystrophin antibody (red signal) and double-labeled with a rat monoclonal antibody for laminin-2 (green). Dystrophin traces are visible in diaphragm from alginate ZM2-M23D oral treated mice. No dystrophin labeling was detected at the sarcolemma in mice untreated or treated with ZM2-AON mdx. (Scale bar = 50 μm).