Fig. 3.
High-performance liquid chromatography-mass spectrometry (LC-MS) of latanoprost samples. Media containing latanoprost released over 1 day from a CL85:15, 45 demonstrated ion chromatograms (A) and annotated high-resolution mass spectra (B) that were substantially similar to those of unencapsulated commercial latanoprost. The mass spectra of both samples generated the same fragmentation pattern of the protonated ion [M + H]+ at m/z 433.29 for latanoprost, and produced abundant fragment ions at m/z 415.28 and 455.27, corresponding to a loss of water [M-H2O + H]+ and a sodium adduct [M + Na]+, respectively. m/z = mass to charge ratio.