Figure 5.

Interaction of wild-type and mutant forms of ghA, ghB, and ghC with human CRP. The microtiter wells were coated with 1 µg/well of CRP. After blocking and washing, different quantities (0.063, 0.125, 0.25, 0.5, 1.0, and 2 µg/well) of (a) ghB, Arg^B114Glu, Arg^B129Ala, and Arg^B163Glu; (b) ghB, His^B117Asp, Lys^B136Glu, and Tyr^B175Leu; and (c) ghC, Lys^C170Glu, Arg^C156Ala, and His^C101Ala were added to the wells and incubated. Mouse anti-MBP antibodies, followed by the rabbit anti-mouse IgG–HRP conjugate detected the amount of bound globular-head modules or their mutants. The data shown is the mean ± SD of triplicate measurements. The result is presented in % residual binding. The values for the wild-type modules were taken as 100%.