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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2013 Nov 7;274(1):10.1016/j.taap.2013.10.029. doi: 10.1016/j.taap.2013.10.029

Fig.5.

Fig.5

A (i)–(iii). α-Smooth muscle actin (α-SMA) immunoreactivity as shown by immunohistochemistry in liver slices from diet-induced obese mouse exposed to bromodichloromethane (DIO+BDCM), diet-induced obese mouse with leptin gene deletion and exposed to bromodichloromethane (Leptin KO) and diet-induced obese mouse with Pfp/Rag2 dual gene deletion and exposed to bromodichloromethane (Pfp/Rag2 dKO) respectively. (iv)–(vi) TGF-β immunoreactivity as shown by immunohistochemistry in liver slices from DIO+BDCM, Leptin KO and Pfp/Rag2 dKO groups respectively. All images were taken in 20× magnification. B. Percentage of α-SMA positive and TGF-β positive immunoreactive cells (obtained by morphometry done on images from three separate microscopic fields in each case) in DIO+BDCM, Leptin KO and Pfp/Rag2 dKO groups compared to DIO group (* P<0.05). C (i)–(iii). Macro- and micro-vescicular collagen deposition as shown by Picrosirius red staining in liver slices from DIO+BDCM, Leptin KO and Pfp/Rag2 dKO groups respectively. Images were taken in 10× magnification. D (i)–(ii). Number of apoptotic nuclei as shown by TUNEL staining in liver slices from DIO+BDCM and Leptin KO groups respectively. Number of TUNEL positive cells identified by their blue-stained nuclei as against the nuclear fast red stained nuclei correspond to the number of apoptotic events. Images were taken in 20× magnification. E. Percentage of TUNEL positive cells (obtained by morphometry done on images from three separate microscopic fields) in DIO+BDCM and Leptin KO groups (* P<0.05). F. mRNA expressions of Bcl-2, Caspase-3 (Casp-3) and P53 from liver homogenates of DIO+BDCM and Leptin KO groups. mRNA expressions had been assessed by quantitative real-time PCR (qRTPCR) and expressions in DIO+BDCM and Leptin KO groups were normalized against mRNA expression in DIO group (* P<0.05). G. Western Blot analysis of pro-caspase-3 and active-caspase-3 (large and small subunits) protein expression levels in liver homogenates from DIO+BDCM and Leptin KO groups. The corresponding β-actin levels are shown in the lower panel.