Figure 4.

Methylation of individual CpG sites from −70 to +39 bp from the transcription start site in the promoter region of Ar is shown for each age in females (top) and males (bottom) for each treatment group. The one significant age × treatment interaction is shown (−63 bp in males). Methylation was positively correlated with expression of Ar mRNA at the following CpGs: in females, −65 bp (EB), −57 bp (EB and A1221), −51 bp (EB), and −5 bp (A1221); and in males, −57 bp (DMSO). Methylation was negatively correlated with Ar mRNA levels at −63 bp (EB) in males. R values and P values for correlation results are shown.