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. 2013 Dec 2;28(1):16–27. doi: 10.1210/me.2013-1165

Figure 5.

Figure 5.

Mature KISS1R is resistant to rapid degradation. HEK-myc-KISS1R cells were pretreated with (A) or without (B) CHX for 10 minutes and then incubated with or without 10−7 M KP10 for the times indicated. The lysates were analyzed by Western blotting using an anti-myc antibody. A representative blot of 3 independent experiments with similar results is shown. C, HEK-myc-KISS1R cells were biotinylated and incubated in the presence or absence of 10−7 M KP10 for 4 or 24 hours. The cells were lysed and immunoprecipitated (IP) with an anti-myc antibody. The immunoprecipitant was analyzed by Western blotting (IB) using HRP-streptavidin (top panel) or HRP-anti-myc antibody (bottom panel). A representative blot of 3 independent experiments with similar results is shown.