FIGURE 7.

Treatment with CNTF or C16 reduced the loss of neurons both in the spinal cord and brain. Nissl staining, bar = 100 μm. [(A,C,E,G,I): coronal sections of brain motor cortex; (B,D,F,H,J): traverse sections through anterior horn of lumbar spinal cord]. At week 8 post-immunization, normal animals (A,B), vehicle-treated EAE rats (C,D), CNTF-treated EAE rats (E,F), C16-treated EAE rats (G,H), and C16 +CNTF-treated EAE rats (I,J). (K,L) The CNTF-, C16-, and C16+CNTF- treated EAE rats all gained more neurons in the motor cortex, and anterior horn of the spinal cord. Neuron counts were restricted to the neurons with a well-defined nucleolus and a cell body with adequate amounts of endoplasmic reticulum, in three visual fields/per section with 200× magnification under bright field viewing. Surviving neural cells (each group % of the normal control) in different groups at week 2 (K) and week 8 (L) post-immunization, calculated after Nissl staining. ^aP < 0.05 versus the normal control; ^bP < 0.05 versus vehicle-treated EAE rats.