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. 2014 Jan;143(1):105–118. doi: 10.1085/jgp.201311053

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© 2014 Bonifacio et al.

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Figure 4. — Kinetic analysis of the ΔF onset. (A) Schematic view illustrating in blue that in A–C, the onset kinetics of ΔF and current are compared. (B) Scatter plot summarizing ΔF onset (red) and current onset (black) rise times measured at pH 6. (C) Current and ΔF trace of a representative experiment with E355C, focusing on the rapid component of the ΔF signal. The vertical dashed line marks the beginning of the current appearance. (D) Schematic view illustrating in blue that in D–G, the ΔF onset and current decay kinetics are compared. (E) Summary graph of ΔF onset rise time (red) and current decay time (black) obtained at pH 6. Mutants whose ΔF is linked to desensitization (i.e., their ΔF rise time and current decay time are not statistically different [ANOVA and Tukey post-hoc test; P > 0.05], and their ratio is within the limit of 1.4-fold) are labeled in blue. (F) Current and ΔF trace of a representative experiment with D78C. The vertical dashed line marks the beginning of the current appearance. (G) Comparison of ΔF rise time (red circles) and the current decay time (black squares) of D78C at different pH. Error bars represent SEM. For B and E, n = 4–12; for G, n = 4.