Figure 5.

Isometric muscle twitch protocol simulates low-intensity exercise. (A) Schematic of the muscle twitching protocol used for in situ TA muscle. Arrows indicate time points of floating microelectrode recordings at baseline (with a single twitch to elicit an action potential) and between 5 and 7 min of repetitive twitching at 1 Hz, with and without superfusion of glyburide. (B) Representative force tracings resulting from isometric twitches of the TA muscle in TG and KO mice with their respective FVB WT and C57BL/6 WT controls.