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. Author manuscript; available in PMC: 2013 Dec 30.
Published in final edited form as: Arch Biochem Biophys. 2012 May 1;523(2):10.1016/j.abb.2012.04.020. doi: 10.1016/j.abb.2012.04.020

Figure 1.

Figure 1

Calpain 10 is downregulated and calpain 10 substrates accumulate in STZ rats four weeks after administration. A, Rat kidney cortex was immunoblotted for calpain 10 (75 kDa), calpain 1 (80 kDa), calpain 2 (80 kDa), and β-actin (42 kDa – loading control). B, PCR reactions were performed with cDNA for calpain 10, calpain 1 and for β-actin. C, Rat kidney cortex was immunoblotted for NDUFB8 (18 kDa), ATP synthase β (56 kDa). D, Rat kidney cortex was immunoblotted for Lon (100 kDa) and GAPDH (37 kDa – loading control). All blots were quantified by densitometric analysis. Data are represented as means ± SEM of % of control (N ≥ 3 for each group). Bars with * are statistically different from control and # are statistically different from STZ + Insulin (p ≤ 0.05).