Table 2.
Primer sequences for real-time reverse transcription-PCR 1
| Gene | (5’–3’) | Accession number 2 |
|---|---|---|
| ALP (Forward) |
GACATGACCTCCCAGGAAGA |
XM_001504312.1 |
| ALP (Reverse) |
GCAGTGAAGGGCTTCTTGTC |
|
| GAPDH (Forward) |
ATCACTGCCACCCAGAAGAC |
NM_001163856 |
| GAPDH (Reverse) |
GTGAGCTTCCCATTCAGCTC |
|
| Osteocalcin (Forward) |
GTGCAGAGTCTGGCAGAGGT |
XM_001915727.1 |
| Osteocalcin (Reverse) |
CCAGCCAATGATCCAGGTAG |
|
| Osterix (Forward) |
GCTCACTATGGCTCCAGTCC |
XM_001494930.3 |
| Osterix (Reverse) |
AAGGTCACTGCCCACAGAGT |
|
| Runx2 (Forward) |
CAGACCAGCAGCACTCCATA |
XM_001502519.3 |
| Runx2 (Reverse) |
GCAGCATTCTGGAAGGAGAC |
|
| Type I Collagen (Forward) |
TTGACCCTAACCAAGGATGC |
AB070840.1 |
| Type I Collagen (Reverse) |
TTCTTGGCTGGGATGTTTTC |
|
| T-box3 (Forward) |
GCATCCCTTTCTCGTCTCTG |
XM_001914978 |
| T-box3 (Reverse) | GACCATCTCGGTACCCCTCT |
1ALP = alkaline phosphatase; GAPDH = glyceraldehyde 3-phosphate dehydrogenase.
Runx2 = runt-related transcription factor 2.
2All sequences from NCBI.