Table 6.
Comparison of liver tissue gene expression levels between microarray and qRT-PCR
| |
Microarray |
Real-time PCR |
|---|---|---|
| Genes | Fold changes in WRR vs. XH | Fold changes in WRR vs. XH |
|
SULT1B1 |
4,13 |
3,28 |
|
LPL |
2,5 |
2,48 |
|
ELOVL6 |
2,18 |
1,76 |
|
ACSBG2 |
5,3 |
2,29 |
|
PNPLA3 |
3,03 |
4,6 |
|
BEAN |
-4,2 |
-5,76 |
|
SLC31A1 |
7,3 |
1,09 |
|
DDT |
-6,59 |
-0,4 |
| PLA2G12A | -2,8 | -2,6 |
Validation of differentially expressed genes between WRR and XH chickens by RT-PCR.
The data presented indicate the relative mRNA expression of both microarray and qRT-PCR.
Positive values mean that the gene was up-regulated when WRR chickens were compared with XH chickens. Similarly, a negative number means that the gene was down-regulated.