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. 2013 Oct 22;13:101. doi: 10.1186/1475-2867-13-101

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Copyright © 2013 Petrini et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HOXB1 promoter methylation in HL60 versus granulocytes and monocytes. Relative percentage of DNA hypermethylated (HM), not methylated (UM), and intermediate methylated (IM) fractions (a). HM and UM in 5-AzaC 1 μM treated HL60 cells after 48 h and 72 h (b). RT-PCR evaluation of HOXB1 in 5-AzaC- and TSA-treated HL60 cells compared with lymphocytes, granulocytes and monocytes (c). GAPDH is shown as quantitative standard. Western blotting of HDAC4 and HOXB1 in TSA-treated HL60 cells (d). Actin was used as quantitative standard.