Table 1.
Differentially expressed genes evaluated by macroarray in HL60/HOXB1 vs HL60/LXSN
| Gene | GenBankID | Function | Ratio HOXB1/LXSN | qRT-PCR ratio |
|---|---|---|---|---|
|
BRCA2 |
U43746 |
Oncogenes & tumor |
0,10 |
|
|
CCNI |
D50310 |
Cell cycle regulators |
0,34 |
|
|
EGR1 |
X52541 |
Transcriptional regulators |
0,21 |
0,02 |
|
FASN |
S80437 |
Fatty acid/Lipid metabolism |
0,13 |
0,25 |
|
FBP2 |
U69126 |
Hydrolases/carbohydrate biosynthesis |
0,50 |
|
|
MDM2 |
Z12020 |
Oncogenes/Apoptosis assoc. proteins |
0,31 |
0,5 |
|
OAS1 |
M11810 |
NucleotidylTransferases |
0,06 |
|
|
SKY |
D17517 |
Protein kinases receptors |
0,58 |
|
|
SOD1 |
K000454 |
Antioxidant/Oxidoreductases |
0,65 |
0,7 |
|
TNFRSF1A |
L41690 |
Growth factor receptors |
0,06 |
|
|
AKAP1 |
X97335 |
Mitochondrial targeting of proteins |
1,94 |
|
|
CASP2 |
U13021 |
Cysteine proteases/Caspases |
1,63 |
1,4 |
|
CCND3 |
M92287 |
Cell cycle regulators |
4,54 |
|
|
CDC37 |
U63131 |
Cell cycle regulators |
7,03 |
|
|
CRM1 |
Y08614 |
Transporter proteins |
2,43 |
|
|
MAPRE1 |
U24166 |
Cytoskeleton Regulators |
1,62 |
|
|
EIF3B |
U78525 |
Initiation of translation factors |
2,49 |
|
|
ERBB3 |
M29366 |
Receptor tyrosine kinases |
3,00 |
|
|
JNK2 |
L31951 |
Intracellular kinase network members |
1,93 |
|
|
KPNB1 |
L38951 |
Transporter proteins |
1,59 |
|
|
NME1 |
X17620 |
Kinases/ Transferases |
2,62 |
1,4 |
|
PDCD10 |
AF022385 |
Apoptosis-associated proteins |
3,50 |
1,4 |
|
PTP4A1 |
U48296 |
Protein tyrosine phosphatases |
2,00 |
|
|
RPS5 |
U14970 |
Ribosomal proteins |
3,00 |
|
|
SPARC |
J03040 |
Matrix-associated proteins |
2,85 |
1,5 |
|
ST13 |
U28918 |
HSC70-interacting proteins |
2,00 |
|
| TRAM | X63679 | Secreted protein translocation | 2,55 |
Differential gene expression was confirmed by RT-PCR when indicated. The reported ratio indicates the observed fold increase or decrease of the HL60/HOXB1 gene expression over HL60/LXSN. Cut-off value 1,6.