Figure 9.

Effect of methionine on osteoblastogenesis in vitro. (A) An in vitro study showing changes in the level of soluble TNFSF11 in methionine pretreated (1–500 μg·mL⁻¹) osteoblast cultures stimulated with LPS endotoxin (1 μg·mL⁻¹). Osteoblast differentiation was induced by adding osteogenic media containing β-glycerophosphate (10 mM) and ascorbic acid (50 μg·mL⁻¹) to pre-osteoblast cultures (cell line passage no. 9). Soluble TNFSF11 in cell culture supernatants was assayed by elisa. (B) Effect of methionine on osteoblast differentiation with or without β-glycerophosphate (10 mM) and ascorbic acid (50 μg·mL⁻¹). Osteoblast differentiation was evaluated by measuring the level of osteocalcin, a bone matrix protein by elisa. Values are mean ± SEM of four independent experiments. *P < 0.05 compared with basal. **P < 0.05 compared with LPS control.