Figure 15.

Effect of siRNA mediated knockdown of MyD88 on TRAP5b and MIP-1α synthesis in developing osteoclast culture. (A) Western blot showing changes in the expression of MyD88 before and after siRNA transfection. siRNA transfections were performed with Lipofectamine RNAiMAX. Cells after stimulation with M-CSF (25 μg·L⁻¹, days 1–11) and TNFSF11 (20 μg·L⁻¹, days 6–20) were transfected either with MyD88 siRNA or scrambled siRNA at concentration of 40 nmol·mL⁻¹. Values are mean ± SEM of four independent experiments. One of the four representative Western blots is given. (B) Changes in the levels of TRAP5b and MIP-1α in response to MyD88 siRNA interference in developing osteoclasts stimulated with M-CSF (25 μg·L⁻¹, days 1–11) and TNFSF11 (20 μg·L⁻¹, days 6–20) ex vivo. Values are mean ± SEM of four independent experiments. *P < 0.05 compared with basal. **P < 0.05 compared with scrambled siRNA.