Figure 6.

The role of HIF1 in the activity of dasatinib. Western blot analysis of the effect of hypoxia (24 h exposure to 0.1% oxygen) and CoCl₂ (6 h exposure to 150 μM and 24 h exposure to 100 μM CoCl₂ under aerobic conditions) on the expression of HIF1α is presented in panel (A). The values presented below each lane represent the ratio of HIF1α to β-actin expression as determined by densitometry. The response of cells to dasatinib in the absence and presence of CoCl₂ is presented in panel (B). Cells were pretreated with 150 μM CoCl₂ for 6 h before a 1 h exposure to dasatinib and 150 μM CoCl₂. Each value presented represents the mean ± SD for three independent experiments. Statistical significance (P < 0.01) between the control (no CoCl₂) and the CoCl₂-treated groups is indicated by *.