Figure 2.
Classification of GABAergic inputs. Human dorsolateral prefrontal cortex (DLPFC) multi-labeled for glutamic acid decarboxylase 65 (GAD65), parvalbumin (PV) and the GABAA α1 subunit. (a1–a3) Single channel and (a4) merged images. (b1–b4) 3X zoom of the boxed region in a1– a4. (c1–c3) Object masks of immunoreactive puncta in b1–b3. (c4) Overlapping object masks marking the parvalbumin basket cell (PVBC) input in b4. In each panel, filled arrowheads indicate PVBC inputs; open arrowheads indicate PV-negative GABAergic inputs, which were excluded from analyses. Scale bar: 2 μm.