Figure 2.

Effect of acidity on tumor cells growth. DU145 (A), PC3 (B) and HPFs (C) were labeled with CFDA-SE and then plated in single culture and in coculture. The cells were grown at various extracellular pH in DME supplemented with 25 mM hepes and 10 mM bicarbonate at 37°C and 5% CO₂. After 40 hours cells were detached and analyzed by flow cytometry. The data show the fold increase of proliferation index calculated on an average of five experiments (*, p < 0.1; **, p < 0.05 and ***, p < 0.01).