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. 2014 Jan 1;127(1):33–39. doi: 10.1242/jcs.123885

Fig. 4.

Fig. 4.

Overexpression of DOA227 but not its kinase-dead mutant inhibits organelle transport. (A) DOA227 (227) or its kinase-dead version (Lys1728Arg; 227KR) were tagged with GFP and overexpressed in S2 cells. Only the catalytically active form inhibits lysosome transport. (B) Effect of DOA knockdown on lysosome motility can be reversed by the wild-type but not kinase-dead DOA227. DOA knockdown was produced using dsRNA against the 3′-UTR region common to all DOA isoforms; overexpression was induced by transfection with cDNA encoding GFP-tagged DOA227 or its catalytically inactive version. Bars in A and B show the relative numbers of vectors larger than 0.2 µm (as a percentage of the control).