Skip to main content
. 2014 Jan 1;127(1):137–146. doi: 10.1242/jcs.133553

Fig. 2.

Fig. 2.

14-3-3θ is required for the interaction between Cx43 and integrin α5. (A) Knockdown of 14-3-3θ expression by a siRNA. MLO-Y4 cells were treated with 14-3-3θ siRNA (lanes 5–7), siRNA from a scrambled sequence (SC; lane 4), only the transfection reagent (Vehicle; lane 3) or were left untreated as the control (C) (lane 2). Lysates of PC12 cells, known to have high 14-3-3θ expression (lane 1) or MLO-Y4 cells were immunoblotted with anti-14-3-3θ, Cx43, integrin α5 or GAPDH antibody. The ratio of the band intensity of 14-3-3θ (left lower panel), Cx43 (right upper panel) or integrin α5 (right lower panel) to GAPDH was quantified. n = 3. ***P<0.001 for 14-3-3θ siRNA versus vehicle and SC (left lower panel). (B) Knockdown of 14-3-3θ disrupted the association between Cx43 and integrin α5. Lysates of MLO-Y4 cells transfected with 14-3-3θ siRNA (lanes 3 and 4) was immunoprecipitated with anti-integrin α5 antibody. Eluates from beads were immunoblotted with anti-integrin α5 or Cx43 antibody. Beads incubated only with integrin α5 antibody (lane 1) or lysates (lane 2) served as negative controls.