Fig. 6.

Loss of 14-3-3θ attenuates the effect of FFSS on promoting the exit of Cx43 from the Golgi. (A) FFSS increases the exit of Cx43 from Golgi. MLO-Y4 cells subjected to FFSS (FF; 16 dynes/cm²) for 2 hours were fixed and dual-immunostained with antibodies against Cx43 and 58K Golgi marker protein. The colocalization is shown in the merged images. The overlap of fluorescence signals were calculated using ImageJ software and a correlation coefficient (Rr) value was obtained that describes the extent of overlap of the two colors. Scale bar: 10 µm. **P<0.01 for FF versus control; n = 3. (B) 14-3-3θ knockdown blocks the increased Golgi exit of Cx43 induced by FFSS. MLO-Y4 cells treated with 30 nM 14-3-3θ siRNA or transfection reagent (vehicle) were subjected to FFSS (16 dynes/cm²) for 2 hours and fixed cells were dual-immunostained with antibodies against Cx43 and 58K Golgi marker protein. The colocalization is shown in the merged images. The overlap of fluorescence signals was calculated using ImageJ software and a correlation coefficient (Rr) value was obtained. Scale bar: 10 µm. **P<0.01 for FF, vehicle versus 14-3-3θ siRNA treatment; n = 3.