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Golgi cisternae can undergo homotypic fusion. (A) Frames from a 4D movie (supplementary material Movie 4) illustrate a homotypic fusion event in an arf1Δ cell. The arrowheads mark two GFP–Vrg4-labeled early Golgi cisternae that fused before maturing into a Sec7–DsRed-labeled late Golgi cisterna. Time is shown in minutes:seconds. Scale bar: 1 µm. (B) The average number of homotypic fusion events per minute for early and late Golgi cisternae in wild-type and arf1Δ strains.
