Fig. 5.
Results of the CTL and ELISPOT IFN-γ-releasing lymphocyte assays. a CTL assays: in vitro cytotoxicity of splenocytes obtained from mice treated with PBS (the control) or with GM3NPhAc-KLH toward FBL3 cells incubated with ManNPhAc. CTL responses were expressed as cell lysis rates determined by the LDH assay. *P < 0.001. b ELISPOT assays of the number of IFN-γ-releasing lymphocytes among 1 × 106 splenocytes derived from mice treated with PBS, GM3NPhAc-KLH plus PBS, and GM3NPhAc-KLH plus ManNPhAc, respectively. *P < 0.05, when compared with the control