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. Author manuscript; available in PMC: 2014 Sep 23.
Published in final edited form as: Chembiochem. 2013 Jun 28;14(14):10.1002/cbic.201300244. doi: 10.1002/cbic.201300244

Figure 2.

Figure 2

Effect of pressure on 1HN chemical shifts. (A) Pressure dependence of the 1HN chemical shifts of N-acetylated α-synuclein measured through a series of 15N-TROSY-HSQC experiments, recorded at 500 MHz, 288 K, and pressures ranging from 1 to 2500 bar. The changes of the 1HN chemical shifts with pressure (δHN(p)) are fitted to a quadratic function: δHN(p) = δ0(p0) + B1(p-p0) + B2(p-p0)2. (B) The second order coefficients (B2) are displayed as a function of the protein sequence (black) and compared with those predicted for residues of the same type when embedded in the model tetrapeptide Ac-G-G-X-A-NH2 (red).[33].