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. Author manuscript; available in PMC: 2014 Jul 23.
Published in final edited form as: Circulation. 2013 Jun 19;128(4):10.1161/CIRCULATIONAHA.113.003183. doi: 10.1161/CIRCULATIONAHA.113.003183

Figure 5.

Figure 5

PI3K regulates TNFα-mediated GRK2 recruitment to the receptor complex. (A) Effect of PI3K inhibition by LY294002 on β2AR phosphorylation and GRK2 recruitment to the plasma membrane following TNFα (n=5). (B) FLAG-β2AR phosphorylation was visualized by confocal microscopy using anti-phospho-β2AR antibody (green) following TNFα in the presence or absence of PI3K inhibitor LY294002. (C) Lysates from HEK-FLAG-β2AR cells following TNFα or vehicle treatment were immunoblotted for PI3Kγ, (n=4).