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Transmission electron microscopy of polyplex interaction with HeLa cells. HeLa cells were treated with polyplexes under serum-free conditions for 1 hr prior to fixation and preparation for TEM. Arrows indicated pHK10 (A,B) and pHK15 (C,D) polyplexes at the plasma membrane (“pm”) in the process of being endocytosed. Scale bar indicates 200 nm.
