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. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: ACS Nano. 2013 Nov 13;7(12):10.1021/nn403069n. doi: 10.1021/nn403069n

Figure 5.

Figure 5

Cellular uptake and transfection efficiency of pHK10 and pHK15 polyplexes in heparan sulfate proteoglycan (HSPG) normal and deficient cells. (A) Cellular uptake of radiolabeled polyplexes. Cells were incubated with 1 µg of [3H]DNA alone, pHK10/[3H]DNA polyplexes, or pHK15/[3H]DNA polyplexes for 4 h under serum-free conditions. The amount of internalized DNA as a function of total DNA (% [3H]DNA internalized) was determined. (B) Transfection of HSPG normal (CHO-K1) and deficient (CHO-pgs-A745) cells. Cells were transfected with DNA (1 µg) at N/P 5 under serum-free conditions. Data are presented mean ± S.D., n = 3. (*) denotes p ≤ 0.05, as determined by a two-tailed, unpaired Student’s t-test with unequal variance.

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