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Transfection efficiency of pHK10 and pHK15 polyplexes in HeLa cells in the presence of endocytic inhibitors. Cells were pre-treated with chlorpromazine, genistein, or amiloride for 1 h prior to the addition of polyplexes formulated with DNA (µ Lg) at N/P 5 under serum-free conditions. Data are presented mean ± S.D., n = 4. (*) denotes p ≤ 0.05, (**) denotes p < 0.01, as determined by a two-tailed, unpaired Student’s t-test with unequal variance.
