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Ensemble-average mean-squared displacement using multiple-particle tracking (MPT). HeLa cells were incubated with Alexa Fluor 568-labeled polymer/DNA complexes for 1 h in serum-free media, rinsed, and then allowed to incubate in complete media for an additional 2 h. Afterwards, 20 s videos of particle trajectories were captured. Ensemble-average mean-squared displacement of PLL (n = 87 in 6 cells), pHK10 (n = 139 in 5 cells), pHK15 (n = 154 in 5 cells) particles.
