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. Author manuscript; available in PMC: 2013 Dec 30.
Published in final edited form as: Methods Mol Biol. 2009;464:10.1007/978-1-60327-461-6_7. doi: 10.1007/978-1-60327-461-6_7

Figure 1.

Figure 1

Schematic representation of the chromosome conformation capture (3C) technology. Formaldehyde is used to treat either yeast or human cells resulting in covalent crosslinks between interacting DNA fragments via protein interactions (indicated by gray square and black circle). The crosslinked cells are then digested, followed by ligation under dilute DNA concentrations. The crosslinks are then reversed and the DNA is purified. The PCR products are detected (PCR primers indicated by arrows) and can then be quantified by gel electrophoresis.