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. Author manuscript; available in PMC: 2013 Dec 30.
Published in final edited form as: Mol Cancer Ther. 2010 May 25;9(6):10.1158/1535-7163.MCT-09-0880. doi: 10.1158/1535-7163.MCT-09-0880

Figure 5.

Figure 5

Overexpression of sCLU overcomes API-2-induced chemosensitivity to docetaxel. A, DU145-DR tumor cells were transfected with either control PCDNA3 or myc-tagged sCLU. These cells were then pretreated with API-2 for 2 h and incubated in docetaxel medium for 48 h. Western blot analysis with anti-myc was done to confirm efficient transfection, and further analysis of sCLU, pAKT, and total AKT was pursued. It is confirmed that API-2 can suppress AKT function in both PCDNA3- and sCLU-expressing tumor cells. B, these same cells were also analyzed for cell growth and survival by methylene blue staining. API-2 effectively induced docetaxel sensitivity and cell death in DU145-DR tumor cells but could not do so in the sCLU-overexpressing DU145-DR tumor cells.