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. Author manuscript; available in PMC: 2014 May 26.
Published in final edited form as: Nat Commun. 2013;4:2841. doi: 10.1038/ncomms3841

Figure 4. Heterochromatin proteins are found in proximity to nascent DNA in defined order following replication.

Figure 4

Antibodies against Drosophila HP1 protein (a), H3K9 demethylase Su(Var)3–9 (b) and H3K9me3 (d) were used for PLA with PCNA (left column) and for CAA with EdU-labeled DNA (columns 2–5). Red arrows indicate when proteins are first detected in these experiments. A scheme of proposed sequence of formation of heterochromatin is shown in d. The results are from three independent experiments. Scale bar, 10 µm.