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Antibodies against Drosophila chromatin remodelers ISWI (a), BRM (b), Polybromo (c) and OSA (d) were used for PLA with PCNA (left column) and for CAA with EdU-labeled DNA (columns 2–5). ISWI, BRM and Plolybromo are first detected on DNA labeled with EdU for 10 min. OSA is first detected on DNA labeled for 2 hr. The results are from three independent experiments. Scale bar, 10 µm.
