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. Author manuscript; available in PMC: 2013 Dec 30.
Published in final edited form as: Toxicon. 2012 Mar 21;60(1):10.1016/j.toxicon.2012.03.011. doi: 10.1016/j.toxicon.2012.03.011

Fig. 4.

Fig. 4

Inhibition of HT-1080, SK-MEL-28, T24 and MDA-MB-231 cells invasion in vitro by r-viridistatin 2. Tumor cells (7.5 × 105 cells/mL, 0.2 mL) were treated with 6.3 μM of r-viridistatin 2 for 30 min at 37 °C, and then placed in the upper Boyden chamber containing a matrigel-coated filter membrane. Invasion was induced by adding McCoy’s 5A minimum essential medium, Eagle’s minimum essential medium and RPMI-1640 medium with FBS to the lower chamber for 24 h. After fixation and removal of non-migrated cells, the cells that migrated to the underside of filter membrane were quantified by light microscopy (3 fields/40×). *p < 0.05 (r-disintegrins compared to negative control).