Fig. 3.

Detection of trans-splicing of exon 1 of the PTM to exons 2 and 3 of the minigene. HEK293 cells were co-transfected with the minigene and PTM constructs and RNA was isolated 48 h later. RT-PCR was performed with PTM-specific F2 forward primer and one of the minigene-specific reverse primers, R1–R5. A specific trans-spliced product of the expected size was detected using reverse primers for exon 2 (R1, R2) and exon 3 (R3–R5)