Fig. 7.

Viral delivery of the PTM. a HEK293 cells were transiently transfected with the minigene plasmid, followed by transduction with a lentivirus harboring the PTM and an eGFP module (MOI: 25). Identical amounts of virus were administered to all wells. After 48 h, the cells were harvested and RNA was isolated for RT-PCR. Specific primer pairs were used to amplify GAPDH, minigene, and trans-spliced product. Duplicate reactions are shown. b Viral transduction (MOI: 50, 25, 12.5, 6, 2.5) was performed in the absence of the minigene. Trans-splicing to the endogenous HTT pre-mRNA of HEK293 cells was proportional to the amount of virus added. M mock transduction (no virus added)