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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nat Protoc. 2013 Jul 11;8(8):10.1038/nprot.2013.084. doi: 10.1038/nprot.2013.084

Figure 6. Strand-specific library types.

Figure 6

The left (/1) and right (/2) sequencing reads are depicted according to their orientations relative to the sense strand of a transcript sequence. The strand-specific library type (F, R, FR, or RF) depends on the library construction protocol and is user-specified to Trinity via the ‘--SS_lib_type’ parameter.