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. Author manuscript; available in PMC: 2013 Dec 30.
Published in final edited form as: Exp Cell Res. 2012 Mar 8;318(8):10.1016/j.yexcr.2012.03.001. doi: 10.1016/j.yexcr.2012.03.001

Figure 5. Effect of insulin on steady-state mRNA levels of CTCF, Pax6 and pro-glucagon in pancreatic islet α cells.

Figure 5

(A) Steady-state mRNA level of CTCF. (B) Steady-state mRNA level of Pax6. (C) Steady-state mRNA level of pro-glucagon. Islet α-cells were starved and treated with insulin (5μg/ml) for different periods of time as indicated. Cells were lysed and RNAs were purified with Trizol. Reverse-transcription was performed and cDNAs were used for qPCR with primer sets for CTCF, Pax6 and pro-glucagon. Symbol “*” represents significant differences in the absence and presence of insulin stimulation (p<0.05, n=6).