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. Author manuscript; available in PMC: 2014 Nov 4.
Published in final edited form as: Vaccine. 2013 Sep 16;31(46):10.1016/j.vaccine.2013.09.010. doi: 10.1016/j.vaccine.2013.09.010

Figure 3. Comparison of Ab responses induced by immunization with gp120/654 made with whole IgG or F(ab′)2.

Figure 3

BALB/c mice were immunized with gp120LAI alone or in complex with intact IgG or F(ab′)2 fragment of mAb 654. F(ab′)2 was produced by pepsin digestion in 20 mM acetate buffer (pH 4.0) at 37°C for 4 hrs and purified by size exclusion chromatography. Sera from mice immunized with PBS and the MPL/DDA adjuvant were tested for control. (A) Sera were collected two weeks after the last immunization, pooled, diluted serially, and tested for neutralization against HIV-1 HXB-2 with the TZM-bl target cells. (B–C) Sera were also tested in ELISA for IgG reactivity to V3HXB-2 peptide (B) and gp120LAI (C). Data from one of two independent experiments are shown. OD405, optical density at 405 nm. * p<0.05 as compared to gp120 and ctrl.