FIG. 4.

Alizarin Red staining and collagen orientation in implant sections: (A, C) show representative images of Alizarin Red stained sections from control (A) and ECM scaffold (C) implant sections. Note the increased calcium deposition in (C). The arrow in (C) represents increased staining observed at the periphery. (B, D) show representative images of collagen fibril orientation in control (B) and ECM scaffold (D) implant sections. Note the presence of polarized collagen at the periphery of the scaffold in (D) (arrow in 4D) and its absence in (B). Color images available online at www.liebertpub.com/tea